HPLC, LC–MS and method validation guides
Work through chromatograms, mass spectra and validation records to see what each analytical method can tell you about a sample.
How to Read an HPLC Chromatogram: Retention, Area and Resolution
Read two synthetic HPLC traces, find retention times and integration bases, and compare overlapping peaks with a worked resolution example.
How to Read a Peptide LC–MS Result: m/z, Charge and Intact Mass
Check ion charge, adducts and mass error, then judge what the evidence supports about peptide identity.
What Analytical Method Validation Shows for an Intended Use
Assess a validation summary by its purpose, sample matrix, reportable range and demonstrated performance.